diff --git a/python/helpers/pydev/_pydevd_bundle/pydevd_pep_669_tracing.py b/python/helpers/pydev/_pydevd_bundle/pydevd_pep_669_tracing.py
index 5513f6cd73c4..122410d61e1d 100644
--- a/python/helpers/pydev/_pydevd_bundle/pydevd_pep_669_tracing.py
+++ b/python/helpers/pydev/_pydevd_bundle/pydevd_pep_669_tracing.py
@@ -35,6 +35,10 @@ try:
 except AttributeError:
     pass
 
+_EVENT_ACTIONS = {
+    "ADD": lambda x, y: x | y,
+    "REMOVE": lambda x, y: x & ~y,
+}
 
 def _make_frame_cache_key(code):
     return code.co_firstlineno, code.co_name, code.co_filename
@@ -121,6 +125,10 @@ def _should_enable_line_events_for_code(frame, code, filename, info):
                 # will match either global or some function
                 if breakpoint.func_name in ('None', curr_func_name):
                     has_breakpoint_in_frame = True
+                    # New breakpoint was processed -> stop tracing monitoring.events.INSTRUCTION
+                    if getattr(breakpoint, '_not_processed', None):
+                        breakpoint._not_processed = False
+                        _modify_global_events(_EVENT_ACTIONS["REMOVE"], monitoring.events.INSTRUCTION)
                     break
 
                 # Check is f_back has a breakpoint => need register return event
@@ -200,6 +208,7 @@ def enable_pep669_monitoring():
             (monitoring.events.LINE, py_line_callback),
             (monitoring.events.PY_RETURN, py_return_callback),
             (monitoring.events.RAISE, py_raise_callback),
+            (monitoring.events.INSTRUCTION, instruction_callback),
         ):
             monitoring.register_callback(DEBUGGER_ID, event_type, callback)
 
@@ -208,6 +217,20 @@ def enable_pep669_monitoring():
         debugger.is_pep669_monitoring_enabled = True
 
 
+def process_new_breakpoint(breakpoint):
+    breakpoint._not_processed = True
+    _modify_global_events(_EVENT_ACTIONS["ADD"], monitoring.events.INSTRUCTION)
+
+
+def _modify_global_events(action, event):
+    DEBUGGER_ID = monitoring.DEBUGGER_ID
+    if not monitoring.get_tool(DEBUGGER_ID):
+        return
+
+    current_events = monitoring.get_events(DEBUGGER_ID)
+    monitoring.set_events(DEBUGGER_ID, action(current_events, event))
+
+
 def _enable_return_tracing(code):
     local_events = monitoring.get_local_events(monitoring.DEBUGGER_ID, code)
     monitoring.set_local_events(monitoring.DEBUGGER_ID, code,
@@ -220,6 +243,57 @@ def _enable_line_tracing(code):
                                 local_events | monitoring.events.LINE)
 
 
+def instruction_callback(code, instruction_offset):
+    try:
+        py_db = GlobalDebuggerHolder.global_dbg
+    except AttributeError:
+        return
+    if py_db is None:
+        return monitoring.DISABLE
+
+    frame = _getframe(1)
+    # print('ENTER: INSTRUCTION ', code.co_filename, frame.f_lineno, code.co_name)
+
+    try:
+        if py_db._finish_debugging_session:
+            return monitoring.DISABLE
+
+        thread = get_current_thread()
+
+        if not is_thread_alive(thread):
+            return
+
+        frame_cache_key = _make_frame_cache_key(code)
+
+        info = _get_additional_info(thread)
+        pydev_step_cmd = info.pydev_step_cmd
+        is_stepping = pydev_step_cmd != -1
+
+        if not is_stepping and frame_cache_key in global_cache_skips:
+            return
+
+        abs_path_real_path_and_base = _get_abs_path_real_path_and_base_from_frame(frame)
+        filename = abs_path_real_path_and_base[1]
+
+        breakpoints_for_file = (py_db.breakpoints.get(filename)
+                                or py_db.has_plugin_line_breaks)
+        if not breakpoints_for_file and not is_stepping:
+            return
+
+        if _should_enable_line_events_for_code(frame, code, filename, info):
+            _enable_line_tracing(code)
+            _enable_return_tracing(code)
+    except SystemExit:
+        return monitoring.DISABLE
+    except Exception:
+        try:
+            if traceback is not None:
+                traceback.print_exc()
+        except:
+            pass
+        return monitoring.DISABLE
+
+
 def py_start_callback(code, instruction_offset):
     try:
         py_db = GlobalDebuggerHolder.global_dbg
diff --git a/python/helpers/pydev/_pydevd_bundle/pydevd_process_net_command.py b/python/helpers/pydev/_pydevd_bundle/pydevd_process_net_command.py
index cc329a67026d..1f7b898e67c2 100644
--- a/python/helpers/pydev/_pydevd_bundle/pydevd_process_net_command.py
+++ b/python/helpers/pydev/_pydevd_bundle/pydevd_process_net_command.py
@@ -8,6 +8,7 @@ import pydevd_tracing
 from _pydev_bundle import pydev_log
 from _pydev_imps._pydev_saved_modules import threading
 from _pydevd_bundle import pydevd_traceproperty, pydevd_dont_trace, pydevd_utils
+from _pydevd_bundle.pydevd_pep_669_tracing import process_new_breakpoint
 from _pydevd_bundle.pydevd_additional_thread_info import set_additional_thread_info
 from _pydevd_bundle.pydevd_breakpoints import LineBreakpoint, get_exception_class
 from _pydevd_bundle.pydevd_comm import (CMD_RUN, CMD_VERSION, CMD_LIST_THREADS,
@@ -452,6 +453,10 @@ def process_net_command(py_db, cmd_id, seq, text):
 
                 id_to_pybreakpoint[breakpoint_id] = breakpoint
                 py_db.consolidate_breakpoints(file, id_to_pybreakpoint, breakpoints)
+
+                if py_db.is_pep669_monitoring_enabled:
+                    process_new_breakpoint(breakpoint)
+
                 if py_db.plugin is not None:
                     py_db.has_plugin_line_breaks = py_db.plugin.has_line_breaks()
                     if py_db.has_plugin_line_breaks:
diff --git a/python/testData/debug/test_add_breakpoint_after_run.py b/python/testData/debug/test_add_breakpoint_after_run.py
new file mode 100644
index 000000000000..d4644da43343
--- /dev/null
+++ b/python/testData/debug/test_add_breakpoint_after_run.py
@@ -0,0 +1,27 @@
+import time
+
+
+def calculate_sum(a, b):
+    # Use a breakpoint here to inspect the program flow
+    result = a + b
+    return result
+
+
+def greet(name):
+    # Use a breakpoint here to debug the call
+    print(f"Hello, {name}!")
+
+
+if __name__ == "__main__":
+    counter = 0
+
+    while True:
+        print("\nIteration:", counter)  # You can set a breakpoint here while the loop runs
+        num1 = counter
+        num2 = counter + 1
+        sum_result = calculate_sum(num1, num2)  # Step into this function during debugging
+        print(f"The sum of {num1} and {num2} is: {sum_result}")
+        greet("Debugger")  # Also step into this function if needed
+        counter += 1
+        # Add a sleep to slow down the loop and make debugging easier
+        time.sleep(2)
\ No newline at end of file
diff --git a/python/testSrc/com/jetbrains/env/debug/PythonDebuggerAggregatorTest.kt b/python/testSrc/com/jetbrains/env/debug/PythonDebuggerAggregatorTest.kt
index 789bc523353d..9d4a105c664c 100644
--- a/python/testSrc/com/jetbrains/env/debug/PythonDebuggerAggregatorTest.kt
+++ b/python/testSrc/com/jetbrains/env/debug/PythonDebuggerAggregatorTest.kt
@@ -1,16 +1,25 @@
 // Copyright 2000-2024 JetBrains s.r.o. and contributors. Use of this source code is governed by the Apache 2.0 license.
 package com.jetbrains.env.debug
 
+import com.intellij.openapi.projectRoots.Sdk
 import com.intellij.xdebugger.XDebuggerManager
 import com.jetbrains.env.PyEnvTestCase
-import org.junit.Test
+import com.jetbrains.python.run.AbstractPythonRunConfiguration
+import com.jetbrains.python.run.PythonRunConfiguration
 import org.junit.Assert.assertEquals
+import org.junit.Test
 
 class PythonDebuggerAggregatorTest: PyEnvTestCase() {
 
   @Test
   fun testSeveralReturnSignal() {
     runPythonTest(object: PyDebuggerTask("/debug", "test_several_return_signal.py") {
+      override fun createRunConfiguration(sdkHome: String, existingSdk: Sdk?): AbstractPythonRunConfiguration<*> {
+        val runConfiguration = super.createRunConfiguration(sdkHome, existingSdk) as PythonRunConfiguration
+        runConfiguration.envs["PYDEVD_USE_CYTHON"] = "NO"
+        return runConfiguration
+      }
+
       override fun before() {
         toggleBreakpoints(6)
       }
@@ -48,4 +57,29 @@ class PythonDebuggerAggregatorTest: PyEnvTestCase() {
       }
     })
   }
+
+  @Test
+  fun testAddBreakpointAfterRun()
+  {
+    runPythonTest(object: PyDebuggerTask("/debug", "test_add_breakpoint_after_run.py") {
+      override fun createRunConfiguration(sdkHome: String, existingSdk: Sdk?): AbstractPythonRunConfiguration<*> {
+        val runConfiguration = super.createRunConfiguration(sdkHome, existingSdk) as PythonRunConfiguration
+        runConfiguration.envs["PYDEVD_USE_CYTHON"] = "NO"
+        return runConfiguration
+      }
+
+      override fun before() {
+        setWaitForTermination(false)
+        toggleBreakpoints(20)
+      }
+
+      override fun testing() {
+        waitForPause()
+        removeBreakpoint(scriptName, 20)
+        resume()
+        toggleBreakpoints(20)
+        waitForPause()
+      }
+    })
+  }
 }
\ No newline at end of file