diff --git a/python/src/com/jetbrains/python/codeInsight/controlflow/PyControlFlowBuilder.java b/python/src/com/jetbrains/python/codeInsight/controlflow/PyControlFlowBuilder.java
index bf0f8a3ae1c5..da1716e952a1 100644
--- a/python/src/com/jetbrains/python/codeInsight/controlflow/PyControlFlowBuilder.java
+++ b/python/src/com/jetbrains/python/codeInsight/controlflow/PyControlFlowBuilder.java
@@ -730,7 +730,10 @@ public class PyControlFlowBuilder extends PyRecursiveElementVisitor {
         else {
           myBuilder.startNode(condition);
         }
+        final PyTypeAssertionEvaluator assertionEvaluator = new PyTypeAssertionEvaluator();
         condition.accept(this);
+        condition.accept(assertionEvaluator);
+        InstructionBuilder.addAssertInstructions(myBuilder, assertionEvaluator);
 
         // Condition is true for nested "for" and "if" constructs, next startNode() should create a conditional node
         prevCondition = condition;
diff --git a/python/testSrc/com/jetbrains/python/Py3TypeTest.java b/python/testSrc/com/jetbrains/python/Py3TypeTest.java
index cd6a54282c47..20d6ad6db0fa 100644
--- a/python/testSrc/com/jetbrains/python/Py3TypeTest.java
+++ b/python/testSrc/com/jetbrains/python/Py3TypeTest.java
@@ -595,6 +595,19 @@ public class Py3TypeTest extends PyTestCase {
     );
   }
 
+  // PY-24445
+  public void testIsSubclassInsideListComprehension() {
+    doTest("List[Type[A]]",
+           "class A: pass\n" +
+           "expr = [e for e in [] if issubclass(e, A)]");
+  }
+
+  public void testIsInstanceInsideListComprehension() {
+    doTest("List[A]",
+           "class A: pass\n" +
+           "expr = [e for e in [] if isinstance(e, A)]");
+  }
+
   private void doTest(final String expectedType, final String text) {
     myFixture.configureByText(PythonFileType.INSTANCE, text);
     final PyExpression expr = myFixture.findElementByText("expr", PyExpression.class);