diff --git a/python/testSrc/com/jetbrains/env/PyEnvTestSettings.kt b/python/testSrc/com/jetbrains/env/PyEnvTestSettings.kt
index aeea862a4c83..52db22dc5353 100644
--- a/python/testSrc/com/jetbrains/env/PyEnvTestSettings.kt
+++ b/python/testSrc/com/jetbrains/env/PyEnvTestSettings.kt
@@ -1,8 +1,12 @@
 // Copyright 2000-2018 JetBrains s.r.o. Use of this source code is governed by the Apache 2.0 license that can be found in the LICENSE file.
 package com.jetbrains.env
 
+import com.intellij.openapi.application.ApplicationManager
+import com.intellij.openapi.vfs.newvfs.impl.VfsRootAccess
 import com.intellij.testFramework.UsefulTestCase.IS_UNDER_TEAMCITY
 import com.intellij.util.SystemProperties
+import com.jetbrains.env.PyEnvTestSettings.Companion.PATH_TO_TEST_ENV_PYTHON_INTERPRETERS
+import com.jetbrains.env.PyTestEnvVars.entries
 import org.jetbrains.annotations.ApiStatus
 import org.jetbrains.annotations.TestOnly
 import java.io.File
@@ -25,6 +29,12 @@ internal data class PyEnvTestSettings(
 ) {
   private val foldersWithPythons: List<File> = listOfNotNull(folderWithCPythons, folderWithCondas).map { File(it) }
 
+  init {
+    // These files might be accessed in tests either directly or via symlinks created by venv
+    val roots = (foldersWithPythons + (additionalInterpreters.map { it.parentFile })).map { it.toString() }.toTypedArray()
+    VfsRootAccess.allowRootAccess(ApplicationManager.getApplication(), *roots)
+  }
+
   /**
    * Paths to all existing python SDKs
    */